ABSTRACT
Objective To investigate antimicrobial susceptibility profile of clinical isolates in Xinhua Hospital Chongming Branch affiliated to Shanghai Jiaotong University School of Medicine for rational use of antibiotics. Methods WHONET 5.6 software was used to analyze the distribution and susceptibility testing data of clinical isolates. Results A total of 5 278 bacterial isolates were collected from 2015 to 2017. The top three bacterial species were Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, accounting for 24.8％, 16.1％ and 8.4％, respectively. The prevalence of major antibiotic-resistant bacteria was increasing. The prevealence of carbapenem-resistant Enterobacteriaceae (CRE) was 0.8％, 3.5％ and 7.0％ in the 3 years. The prevalence of MRSA was 32.4％, 41.9％, and 51.3％, respectively. The prevalence of the Acinetobacter strains resistant to antibiotics, especially to imipenem and meropenem, increased from 20.3％ to 64.6％. No vancomycin-or linezolid-resistant isolates were found in gram-positive bacteria. Conclusions The clinical bacterial isolates show increasing resistance to most antibiotics during the 3-year period in this secondary care general hospital. Especially, carbapenem-resistant Enterobacteriaceae poses a serious threat. Attention should also be paid to surveillance of antimicrobial resistance in secondary care general hospital.
ABSTRACT
Objective To analyze the antimicrobial resistance profile of clinical isolates in Shanghai Xinhua Hospital Chongming Branch affiliated to Shanghai Jiaotong University School of Medicine , a member of China Antimicrobial Resistance Surveillance System, during 2015, for the purpose to facilitate rational antimicrobial therapy. Methods Strain identification?and?susceptibility?testing?were?carried?out?for?the?clinical?isolates?using?MicroScan?WalkAway?96?Automated?Systems and Kirby-Bauer method. Results In 2015, a total of 1815 isolates were collected, including gram-negative bacteria (73.2 %) and gram-positive bacteria (26.8 %). The top three frequently isolated species were Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. ESBL-producing strains were found in 36.3 % of the Escherichia coli isolates, 12.6 % of the Klebsiella (K. pneumoniae and K. oxytoca) isolates, and 28.0 % of the Proteus mirabilis isolates. The prevalence of carbapenem-resistant strains was 0.69 % in Enterobacteriaceae isolates. The prevalence of methicillin-resistant strain was 29.1 % in S. aureus, and 61.4 % in coagulase-negative Staphylococcus isolates. No more than 15 % of the Enterobacteriaceae isolates and no more than 20 % of the P. aeruginosa and Acinetobacter isolates were resistant to carbapenems. No vancomycin-or linezolid-resistant strains were found in Enterococcus or Staphylococcus. Conclusions Antibiotic-resistant clinical isolates are a serious threat for clinical antimicrobial treatment. We should pay more attention to such urgent situation and rational use of antibiotics.
ABSTRACT
Objective To evaluate Cica-Beta Test kit for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa (PAE)in the clinical microbiology laboratory.Methods A total of 82 imipenem-resistant PAE clinical isolates from litera-ture[5]was dectected to metallo-β-lactamase (MBLs)by PAE-MHT and Cica-Beta Test kit.Results The sensitivity,speci-ficity and accuracy rate of PAE-MHT was 84.6%,97.2% and 97.6%,and the sensitivity,specificity and accuracy rate of Cica-Beta Test kit was 76.9%,100% and 96.3%,respectively.Two methods had a good consistency.Conclusion Two methods are simple,quick for detecting to metallo-β-lactamase-producing Pseudomonas in clinical laboratories.